Environmental contamination of perfluoroalkyl and polyfluoroalkyl substances (PFAS) is of significant concern due to a wide range of adverse health effects induced by these chemicals, including hepatotoxicity. Analyzing next-generation sequencing data (NGS) from RNA-seq experiments to identify PFAS-induced adverse outcome pathways in the liver may help better understand the mechanisms of PFAS-induced hepatotoxicity. This training will use QIAGEN CLC Genomics Workbench to generate differential expression data from RNA-seq and QIAGEN IPA to study the data within a biological context and uncover molecular mechanisms and regulatory molecules.
In this training, you’ll learn how to:
• Import NGS data into QIAGEN CLC Genomics Workbench
• Generate differential expression data using template workflows
• Export data to IPA
• Investigate pathways and regulatory networks to understand biological mechanisms
• Compare different experimental groups to identify biological similarities and differences
• Generate a custom network
Please consider reviewing the below tutorials before this meeting.
QIAGEN CLC: https://qiagen.showpad.com/share/XwwP0oVcMO1WKDGWcEf96
QIAGEN IPA: https://qiagen.showpad.com/share/SQjinvdxIs1iGhL8H3eOd
In this 90-minute training, you'll learn how to perform drug treatment, toxicology and target safety assessment-related discoveries using QIAGEN Ingenuity Pathway Analysis (IPA) and QIAGEN Omicsoft Lands.
Using public data from GTEx (normal tissue), GEO, cancer collections and more, you'll learn how to use Omicsoft Lands to:
• Investigate a drug target or biomarker expression across different normal tissues, disease conditions, treatments and more
• Correlate the expression of two or more genes
• Identify a list of genes or biomarkers specific to treatment, disease, normal tissue, cell type and more
Using findings from peer-reviewed publications and other sources, we'll explore with you how to use QIAGEN IPA to:
• Study the impact of targeting a gene/protein on different toxicological and biological functions
• Derive toxicity findings for a gene of interest from QIAGEN IPA's knowledgebase
• Identify and study toxicity-related pathways, regulators and functions for an internal dataset or a public dataset
• Compare different drug treatments, other conditions or multi-omics data for novel discoveries
With September upon us, the EUROTOX 2015 Congress is just around the corner. Attended by some of the world’s leading toxicologists to share ideas and scientific developments, EUROTOX is probably Europe’s most important toxicology event. This year it takes place at the Alfândega International Congress Center from September 13-16 in beautiful Porto, Portugal, where the city center was declared a UNESCO World Heritage site in 1996.
Porto is known as the “City of Bridges,” which elegantly mirrors this year’s EUROTOX theme, “Bridging Sciences for Safety.” The meeting’s focus will be on the critical role of toxicology in safely bridging scientific knowledge across biology, chemistry, pharmacology, and physiology—which means that the interpretation of large-scale ’omics data is imperative. To help uncover the significance of data and identify new targets or candidate biomarkers within the context of biological systems, toxicologists are turning to Ingenuity® Pathway Analysis, our web-based software application featuring powerful analysis and search tools for gene expression data.
During EUROTOX, QIAGEN Bioinformatics will be at booth #43, where you can learn more about IPA and how it can improve your NGS workflow. In addition to our presence in the exhibit hall, we have two scheduled poster sessions. Also, don’t miss out on the opportunity to meet Dr. Stuart Tugendreich and Dr. Elodie Dubus on September 14 and 15 during the following sessions:
We think you’ll find these sessions to be very compelling, and we invite you to attend. We hope to see you at EUROTOX!
Read more about Ingenuity Pathway Analysis
