Read about how researchers across the world are using QIAGEN Digital Insights solutions to accelerate their work in a variety of applications
Making sense of complex 'omics data, and developing the infrastructure to compile, store, search, analyze and visualize relevant information has significant challenges and may pose a burden to researchers without bioinformatics skills. Yet powerful insights derived from 'omics data help innovate, integrate and translate scientific results into impactful discoveries. Many noteworthy papers cite QIAGEN Digital Insights solutions and demonstrate how our tools help drive research insights and discoveries. These papers use QIAGEN Ingenuity Pathway Analysis (IPA), QIAGEN CLC Genomics and/or QIAGEN OmicSoft to help drive success. The QIAGEN Digital Insights portfolio encompasses a comprehensive, easy-to-use toolbox that ensures continuity in the NGS workflow. Here, we have curated a selection of just a few recent papers to offer a sense of the diversity of the research for which QIAGEN Digital Insights solutions makes a difference.
Multi-organ proteomic landscape of COVID-19 autopsies
First author: Xui Nie
Check out this fantastic work by coronavirus researchers at Westlake University who try to understand how SARS-CoV-2 causes cellular damage in organs other than the lungs. See how the team uses QIAGEN IPA to identify upregulated molecules and activated pathways in this proteomic study of COVID-19 autopsy samples. Read their full paper here.
EGR1 is a gatekeeper of inflammatory enhancers in human macrophages
First author: Marco Trizzino
Monocytes or macrophages? See how the team at The Wistar Institute used QIAGEN IPA to understand how EGR1 drives immune cell differentiation by studying upstream regulators of the genes associated with EGR1. Read the full article here.
Wastewater-based epidemiology as a useful tool to track SARS-CoV-2 and support public health policies at the municipal level in Brazil
First author: Tatiana Prado
We’ve heard wastewater can be used for COVID-19 surveillance. Check out this QIAGEN CLC research paper on wastewater-based epidemiology as a useful tool to track SARS-CoV-2 in Brazil. Read the full article here.
KLF10 deficiency in CD4+ T cells triggers obesity, insulin resistance and fatty liver
First author: Akm Khyrul Wara
Is immune dysregulation and obesity/insulin resistance connected? Researchers at Harvard Medical use QIAGEN IPA and QIAGEN CLC Genomics to study the role of CD4+ T cells in insulin resistance and obesity to reveal how KLF10, a transcription factor and a critical regulator of CD4+ T regulatory cells, maybe be responsible. Read the full article here.
Single-cell analysis reveals distinct immune landscapes in transplant and primary sarcomas that determine response or resistance to immunotherapy
First author: Amy J. Wisdom
Does immunotherapy work differently in primary vs. transplanted tumors? See how cancer researchers at Duke University use QIAGEN OmicSoft Suite for single-cell analysis of immune cells found in transplant vs. primary tumors and how these cells play a role in the effectiveness of immunotherapy. Read the full paper here.
Get in touch with us, we would love to hear from you. To request information on our QIAGEN Digital Insight solutions, contact bioinformaticssales@qiagen.com.
Read about how researchers across the world are using QIAGEN Digital Insights solutions to accelerate their work in a variety of applications
Making sense of complex 'omics data, and developing the infrastructure to compile, store, search, analyze and visualize relevant information has significant challenges and may pose a burden to researchers without bioinformatics skills. Yet powerful insights derived from 'omics data help innovate, integrate and translate scientific results into impactful discoveries. Many noteworthy papers cite QIAGEN Digital Insights solutions and demonstrate how our tools help drive research insights and discoveries. These papers use QIAGEN Ingenuity Pathway Analysis (IPA), QIAGEN CLC and/or QIAGEN OmicSoft to help drive success. The QIAGEN Digital Insights portfolio encompasses a comprehensive, easy-to-use toolbox that ensures continuity in the NGS workflow. Here, we have curated a selection of just a few recent papers to offer a sense of the diversity of the research for which QIAGEN Digital Insights solutions makes a difference.
Aberrant (pro)renin receptor expression induces genomic instability in pancreatic ductal adenocarcinoma through upregulation of SMARCA5/SNF2H
First author: Yuki Shibayama
Did you know on average pancreatic cancer patients acquire over 67 non-synonymous mutations? The team at Kagawa University used QIAGEN IPA to study the role of (pro)renin receptor [(P)RR] in causing genomic instability. Read their full paper here.
Glioblastoma stem cells induce quiescence in surrounding neural stem cells via Notch signaling
First author: Katerina Lawlor
Did you know cancer cells are not only good at proliferating but can also suppress other cells from growing? See how the team at Imperial College London investigates this phenomenon using QIAGEN IPA to understand how cancer cells induce quiescence in glioblastomas. Read their full paper here.
Multiparametric profiling of engineered nanomaterials: Unmasking the surface coating effect
First author: Audrey Gallud
Discover this fascinating research by scientists at the Karolinska Institutet who study the cytotoxic effects of engineered nanomaterials (ENMs). See how the team uses QIAGEN IPA to understand the mechanism behind the cytotoxic effects of ENMs and how to mitigate the risks. Read the full article here.
Innate immune training of granulopoiesis promotes anti-tumor activity
First author: Lydia Kalafati
Check out this exciting research by L. Kalafati and colleagues at TU Dresden, who try to promote the anti-tumor activity of trained neutrophils. See how the team uses QIAGEN IPA to understand the molecular mechanism behind reprogramming caused by trained immunity agonists. Read the paper here.
Liver-expressed cd302 and cr1l limit hepatitis C virus cross-species transmission to mice
First author: Richard J. P. Brown
Did you know the hepatitis C virus (HCV) affects 71 million people worldwide but only infects humans? Read how researchers at Paul Ehrlich Institute (PEI) use QIAGEN IPA and QIAGEN CLC Genomics Workbench to understand how mice are able to prevent HCV infection. Read their full paper here.
Vascular disease and thrombosis in SARS-CoV-2-infected rhesus macaques
First author: Malika Aid
Is there a connection between thrombosis and SARS-CoV-2 infection? Read how the team at Beth Israel Deaconess Medical Center uses QIAGEN IPA to understand the critical interactions between various pathways that lead to SARS-CoV-2-induced blood clotting in rhesus macaques. Read their full paper here.
Imbalance of regulatory and cytotoxic SARS-CoV-2-reactive CD4+ T cells in COVID-19
First author: Benjamin Meckiff
Check out this critical coronavirus research by B. Meckiff and colleagues at the La Jolla Institute for Immunology, who study the role of CD4+ T cells in COVID-19. See how the team uses QIAGEN IPA to understand how different subsets of CD4+ T cells play a role in pathogenic immune responses to SARS-CoV-2 infection. Read the full article here.
Potentially adaptive SARS-CoV-2 mutations discovered with novel spatiotemporal and explainable AI models
First author: Michael R. Garvin
Can mutations in coronavirus spike proteins help it escape current vaccines? See how a group at Oak Ridge National Laboratory predicts mutational hotspots in the viral genome using QIAGEN CLC Genomics and AI models. Read the full article here.
Genomic evidence for reinfection with SARS-CoV-2: A case study
Co-author: Joel Sevinsky
Is SARS-COV-2 reinfection possible? Joel Sevinsky and his colleagues in the Nevada public health arena report the first SARS-Cov-2 reinfection case in the US. See how the team uses QIAGEN CLC Genomics Workbench for bioinformatics analysis of their SARS-CoV-2 samples to discover whether it was the same virus or a genetically different specimen. Read the full article here.
Two distinct immunopathological profiles in autopsy lungs of COVID-19
First author: Ronny Nienhold
Is unlocking differences in immune response the key to treating ARDS in COVID-19? Dig into this important coronavirus research from R. Nienhold and colleagues at Cantonal Hospital Baselland who study different immunopathological profiles in COVID-19 patients. See how the team uses QIAGEN CLC Genomics Workbench to understand the different immune patterns observed in post mortem COVID-19 lung tissue. See their full article here.
A mouse-adapted SARS-CoV-2 induces acute lung injury and mortality in standard laboratory mice
First author: Sarah R. Leist
Did you know coronaviruses are responsible for three epidemics in the 21st century? Great work by S. Leist and colleagues at the University of North Carolina at Chapel Hill, who created a mouse-adapted SARS-CoV-2 to understand the virus better. See how the team uses QIAGEN CLC Genomics Workbench to characterize this animal model and discover mechanisms for SARS-CoV-2 pathogenesis to test potential therapeutics. Read their full paper here.
Single-cell transcriptomics implicate novel monocyte and T cell immune dysregulation in sarcoidosis
First author: Lori Garman
Single-cell analysis improves our understanding of multimodal diseases. Don't miss this exciting cancer research by L. Garman and colleagues, who study the role of immune cells in sarcoidosis. The team uses QIAGEN IPA and QIAGEN OmicSoft DiseaseLand to identify dysregulated pathways using single-cell analysis. Read the full paper here.
Non-human primate blood–brain barrier and in vitro brain endothelium: From transcriptome to the establishment of a new model
First author: Catarina Chaves
Congratulations to the researchers at Sanofi for publishing their findings on a comparative model for the human blood-brain barrier (hBBB). See how the team uses QIAGEN IPA and QIAGEN OmicSoft Studio to investigate the transcriptome of brain capillaries from a non-human primate, and compare it to the hBBB. Read the full paper here.
Preclinical validation of therapeutic targets predicted by tensor factorization on heterogeneous graphs
First author: Saee Paliwal
Do we need better models for validating preclinical drug target candidates? How can we test these models? Read how researchers at BenevolentAI use QIAGEN OmicSoft DiseaseLand to evaluate the robustness of their computational model, Rosalind. Read the full paper here.
Get in touch with us! To request information on our QIAGEN Digital Insight solutions, contact bioinformaticssales@qiagen.com.
Read about how researchers across the world are using QIAGEN Digital Insights solutions to accelerate their work in a variety of applications
Powerful insights help innovate, integrate and translate scientific results into impactful discoveries. Many noteworthy papers cite QIAGEN Digital Insights solutions and demonstrate how our tools help drive research insights and discoveries. These papers use QIAGEN Ingenuity Pathway Analysis (IPA), QIAGEN CLC and/or QIAGEN OmicSoft to help drive success. The QIAGEN Digital Insights portfolio encompasses a comprehensive, easy-to-use toolbox that ensures continuity in NGS workflow. Here, we have curated a selection of just a few recent papers to offer a sense of the diversity of the research for which QIAGEN Digital Insights solutions makes a difference.
Viral cGAMP nuclease reveals the essential role of DNA sensing in protection against acute lethal virus infection
First author: Bruno Hernáez
Check out this fascinating research from Dr. B. Hernáez and colleagues at the Autonomous University of Madrid who investigate the ability of native cells to detect viral infection. Read how the team uses QIAGEN IPA to understand the role of DNA-sensing pathways in cellular detection and protection against acute lethal viral infection. Read their full paper here.
3D curvature-instructed endothelial flow response and tissue vascularization
First author: Christian Mandrycky
Can the way blood flows in three dimensions affect cellular transcriptomics? Read this exciting research by Dr. C. Mandrycky and colleagues at the University of Washington who try to understand the effects of vascularization in a three-dimensional space. Learn how the team uses QIAGEN IPA to understand the single-cell transcriptomic changes that occur in endothelial cells when a spiral blood flow is applied. Read the full article here.
Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis
First author: Alyssa M. Flores
Discover this fascinating nanotechnology research out of Stanford University by Dr. A Flores and colleagues, who investigate phagocytosis as a therapy for clearing atherosclerotic plaques. See how the team uses QIAGEN IPA to show the effectiveness of drug dispensing nanoparticles in reducing pro-inflammatory pathways and potentially preventing atherosclerosis. Read their full paper here.
Elevated calprotectin and abnormal myeloid cell subsets discriminate severe from mild COVID-19
First author: Aymeric Silvin
Don’t miss this critical research by Dr. A. Silvin and colleagues from Inserm, who investigate biomarkers to predict the development of severe COVID-19 in patients. Delve into their study, and learn how QIAGEN IPA supports their understanding of how the accumulation of calprotectin and abnormal monocytes may help differentiate between severe and mild COVID-19. Read the full article here.
Type I and III interferons disrupt lung epithelial repair during recovery from viral infection
First author: Jack Major
Immunologists at the Francis Crick Institute investigate the harmful effects of excessive cytokine signaling during respiratory viral infection. Learn how they use QIAGEN IPA to investigate how IFN signaling interferes with lung repair during influenza/viral recovery. Read their full paper in Science here.
Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and - independent cellular responses in Alzheimer’s disease
First author: Yingyue Zhou
Are we a step closer to understanding Alzheimer’s risk? Learn about fascinating research by Y. Zhou and colleagues at Washington University in St. Louis who study the role of microglia receptor TREM2 in Alzheimer's pathogenesis. See how they use QIAGEN IPA to investigate how TREM2 increases AD risk and activation of disease-associated microglia. Read the full article here.
Elevated glucose levels favor SARS-CoV-2 infection and monocyte response through a HIF-1α/glycolysis-dependent axis
First Author: Ana Campos Codo
Discover why diabetic patients may be more at risk of developing severe COVID-19. Researchers at the Universidade Estadual de Campinas investigate the role of elevated blood glucose levels in inducing cytokine storms in severe COVID-19 patients. See how the team uses QIAGEN IPA to investigate how the HIF-1α axis in monocytes causes T-cell dysfunction and reduces epithelial cell survival. Read their full paper here.
Genomic characterization of SARS-CoV-2 identified in a reemerging COVID-19 outbreak in Beijing's Xinfadi market in 2020
First author: Yong Zhang
Should we be looking for new mutations in SARS-CoV-2 that make it more virulent? Researchers from the Chinese Center for Disease Control and Prevention perform genomic characterization of SARS-CoV-2 identified in a reemerging outbreak in China. Discover how they use QIAGEN CLC Genomics Workbench to help trace the source of the virus in this second outbreak in Beijing’s Xinfadi market. Read their full article here.
Genetic tracing of HCoV-19 for the re-emerging outbreak of COVID-19 in Beijing, China
First author: Jing Yang
Crucial coronavirus research from the Chinese Academy of Sciences looking into the re-emergence of the SARS-CoV-2 virus in China. Discover how they use the nanopore and MiSeq system together with QIAGEN CLC Genomics Workbench to trace the source of the virus in this second outbreak in Beijing. Get the full article here.
Systematic reconstruction of the complete two-component sensorial network in Staphylococcus aureus
First author: B. Rapun-Araiz
High-impact research by B. Rapun-Araiz and colleagues at Universidad Publica de Navarra in Spain who investigate the targets of two-component signal transduction systems (TCSs) in bacteria. See how they use QIAGEN CLC Genomics Workbench to map the complete TCS regulon in Staphylococcus aureus. Read the full paper here.
Remdesivir inhibits SARS-CoV-2 in human lung cells and chimeric SARS-CoV expressing the SARS-CoV-2 RNA polymerase in mice
First author: Andrea J. Pruijssers
Excellent research by A. Pruijssers and colleagues at Vanderbilt University who study how Remdesivir inhibits SARS-CoV-2 in human lung cells. See how they use QIAGEN CLC Main Workbench to help investigate the efficacy of Remdesivir against SARS-CoV-2 in vitro and in vivo. Read the full article here.
Lysosomal recycling of amino acids affects ER quality control
First author: Ryo Higuchi-Sanabria
Exciting research from the Howard Hughes Medical Institute, where researchers investigate the role of lysosomes in amino acid recycling. Learn how they use QIAGEN CLC Genomics Workbench to understand how reduced lysine and arginine can cause increased sensitivity to proteotoxic stress in the endoplasmic reticulum (ER). Read the full paper here.
Liver-specific knockdown of class IIa HDACs has limited efficacy on glucose metabolism but entails severe organ side effects in mice
First author: Nicole Ziegler
Could HDAC inhibitors serve as epigenetic therapy? Intriguing research by Dr. N. Ziegler and colleagues at Sanofi investigate the therapeutic potential of class IIa HDAC inhibition. See how the team uses QIAGEN OmicSoft Studio to understand the consequences of this inhibition, which has a limited effect on glucose metabolism but causes severe side effects in the kidney and spleen. Read the full paper here.
The evolving systemic biomarker milieu in obese ZSF1 rat model of human cardiometabolic syndrome: Characterization of the model and cardioprotective effect of GDF15
First Author: Marina Stolina
Can we develop biomarkers to predict cardiometabolic syndrome using the obese ZSF1 rat model? Read this paper to learn how researchers at Amgen use QIAGEN IPA and OmicSoft Studio to characterize the obese ZSF1 rat model to develop biomarkers that predict cardiometabolic syndrome, a global health issue, to better understand the cardioprotective effects of GDF15. Read the full article here.
Elucidation of mechanisms of topotecan-induced cell death in human breast MCF-7 cancer cells by gene expression analysis
First author: Birandra K. Sinha
Fascinating research by Dr. B Sinha and colleagues at the National Institute of Environmental Health Sciences (NIEHA), who investigate the mechanisms of topotecan-induced cell death in human breast cancer cells. Learn how the team uses QIAGEN OmicSoft Studio to understand the role of reactive oxygen species in inducing tumor cell killing. Read the full paper here.
ABHD11, a new diacylglycerol lipase involved in weight gain regulation
First author: Johanna Escoubet
Exciting discovery by J. Escoubet and colleagues at Sanofi who study a new diacylglycerol lipase involved in weight gain regulation. Learn how they use QIAGEN ArrayStudio to understand how inhibiting ABHD11 helps reduce intestinal fat absorption. Read the full paper here.
To request information on the QIAGEN Digital Insight solutions, contact bioinformaticssales@qiagen.com.
Researchers across the world are using QIAGEN Digital Insights solutions to accelerate their work in a variety of applications
Powerful insights help innovate, integrate and translate scientific results into impactful discoveries. Many noteworthy papers cite QIAGEN Digital Insights solutions and demonstrate how our tools help drive research insights and discoveries. These papers use QIAGEN Ingenuity Pathway Analysis (IPA), QIAGEN CLC and/or QIAGEN OmicSoft to help drive success. The QIAGEN Digital Insights portfolio encompasses a comprehensive, easy-to-use toolbox that ensures continuity in NGS workflow. Here, we have curated a selection of just a few recent papers to offer a sense of the diversity of the research for which QIAGEN Digital Insights solutions makes a difference.
Proteomic and metabolomic characterization of COVID-19 patient sera
First author: Bo Shen
Virologists at Wenzhou Medical University use machine learning to help identify potentially severe COVID19 cases. Learn how they use QIAGEN IPA to understand proteomic and metabolomic changes to identify blood markers that could predict COVID-19 severity. Read their full paper here.
Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface
First author: Joseph Collin
Potential SARS-CoV-2 transmission through the eyes? Discover this fascinating research by Dr. J. Collin and colleagues at Newcastle University, who are studying a possible ocular route of transmission for SARS-CoV-2. Learn how the team uses QIAGEN IPA's Upstream Regulator Analysis to understand how the virus may exploit ACE2 and TMPRSS2 co-expression in the eyes to gain systemic entry. Read the full article here.
Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds
First Author: Mateusz Wietecha
Novel research by Dr. M. Wietecha and colleagues at ETH Zurich who study scar tissue formation during wound healing. Learn how the team uses QIAGEN CLC Genomics Workbench and QIAGEN IPA to understand how activin A affects the wound healing process and could be a key factor in wound fibrosis. Read their full paper here in Nature Communications.
RasGRP1 is a causal factor in the development of l-DOPA–induced dyskinesia in Parkinson’s disease
First author: Mehdi Eshraghi
Parkinson’s researchers at the Scripps Research Institute investigate the role of RasGRP1 in causing L-DOPA–induced dyskinesia. Learn how the team uses QIAGEN IPA to understand the molecular mechanism of how this guanine nucleotide exchange factor contributes to this disease. Read their full paper here.
Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice
First author: Julia Duerr
Researchers at the University of Heidelberg use QIAGEN IPA to investigate the role of Nedd4-2 in progressive idiopathic pulmonary fibrosis. See how QIAGEN IPA helps the team identify processes and pathways affected by treatment with the anti-fibrotic drug pirfenidone in a Nedd4-2 conditional deletion mouse model. Read their full paper in Nature Communications.
Concise whole blood transcriptional signatures for incipient tuberculosis: A systematic review and patient-level pooled meta-analysis
First author: Rishi K. Gupta
Critical tuberculosis research by R. Gupta and colleagues from University College London who investigate transcriptional signatures to identify potential tuberculosis infection in patients. See how the team uses QIAGEN IPA to research blood transcriptional biomarkers that could be used to identify high-risk tuberculosis patients. Read the full paper from Lancet Respiratory Medicine.
Mitochondria-endoplasmic reticulum contacts in reactive astrocytes promote vascular remodeling
First author: Jana Goebel
In honor of brain injury awareness month, we are highlighting fascinating research from the University of Cologne, Germany, where J. Gӧbel and colleagues look at the physical interaction between the endoplasmic reticulum and mitochondria and its role in promoting vascular remodeling in astrocytes. See how the team uses QIAGEN IPA to investigate this mitochondrial dynamic in brain injury wound healing. Read the full paper in Cell Metabolism.
Transcriptional regulation of CCL2 by PARP1 is a driver for invasiveness in breast cancer
First author: Pranabananda Dutta
Dutta and colleagues at Charles Drew University are studying the role of PARP1 in metastatic breast cancer. The team uses QIAGEN CLC Genomics Workbench and QIAGEN IPA to understand how PARP1 interacts with CCL2, a driver for invasiveness in breast cancer. Access their full article here.
Evolution of a new function by fusion between phage DNA and a bacterial gene
First author: Omar Warsi
Discover fascinating research by Dr. Warsi and colleagues at Uppsala University, where they conferred a new function in bacteria by creating a chimeric fusion between phage DNA and a bacterial gene. The team used QIAGEN CLC Genomics Workbench to understand how the fusion of mobile genetic elements generates novel functions in bacteria. Read their full paper here.
SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease
First author: So Ra Kim
Discover exciting research by S. R. Kim and colleagues at Yonsei University who study the role of SGLT2 in modulating NLRP3 inflammasome activity in diabetic patients. They use the powerful QIAGEN CLC Genomics Workbench to investigate how SGLT2 inhibition helps reduce cardiovascular events. Read the full paper in Nature Communications here.
A novel coronavirus from patients with pneumonia in China, 2019
First author: Na Zhu
Exciting coronavirus research by N. Zhu and colleagues at The Chinese Center for Disease Control, who report how they identified the novel coronavirus responsible for COVID-19. Discover how the team uses QIAGEN CLC Genomics Workbench in the data analysis of next-generation sequencing of samples from pneumonia patients in Wuhan, China. Read their New England Journal of Medicine publication here.
Successful generation of epigenetic disease model mice by targeted demethylation of the epigenome
First author: Takuro Horii
Discover this fantastic research by T. Horii and colleagues at Gunma University in Japan, where they are working on creating an epigenetic mouse disease model using targeted methylation of the genome. Learn how the team uses QIAGEN extraction kits and QIAGEN CLC Genomics Workbench to create a Silver-Russell syndrome animal model using targeted demethylation. Read the full paper here.
Metabolic alterations in spheroid-cultured hepatic stellate cells
First author: Koichi Fujisawa
Researchers at Yamaguchi University are studying transcriptomic and metabolomic changes in hepatic stellate cells in spheroid culture to better understand liver fibrosis. Discover how they use QIAGEN OmicSoft Suite and QIAGEN IPA to identify key changes that deepen our understanding of liver fibrosis. Read the full paper here.
Systems biology analysis of the antagonizing effects of HIV-1 TAT expression in the brain over transcriptional changes caused by methamphetamine sensitization
First author: Liana Basova
What's the connection between drug use and certain viruses? Discover this fascinating research by Dr. L. Basova and colleagues at the San Diego Biomedical Research Institute, who investigate the effects of reward circuitry inhibiting the TAT protein in HIV patients using methamphetamines. See how the team uses QIAGEN OmicSoft ArrayStudio together with QIAGEN IPA to understand how TAT has an antagonizing effect on meth-induced transcriptional changes in a neuroHIV mouse model. Read the full paper in Viruses.
Antitumor potency of an anti-CD19 chimeric antigen receptor T-cell therapy, lisocabtagene maraleucel in combination with ibrutinib or acalabrutinib
First author: Jim S. Qin
Cancer researchers at Juno Therapeutics investigate the effectiveness of a new anti-CD19 T-cell therapy. See how the team uses QIAGEN ArrayStudio to research a combination therapy of lisocabtagene maraleucel and ibrutinib or acalabrutinib in hopes to improve outcomes in CD19+ B-cell malignancies. Read the full paper here.
Mitochondrial dysfunction and DNA damage accompany enhanced levels of formaldehyde in cultured primary human fibroblasts
First author: Cristina A. Nadalutti
Researchers at the National Institute of Environmental Health Sciences (NIEHS) use QIAGEN OmicSoft Array Suite to study how increases in cellular formaldehyde cause mitochondrial dysfunction and DNA damage in cultured primary human fibroblasts. Read the full paper here.
To request information on the QIAGEN Digital Insight solutions, contact bioinformaticssales@qiagen.com.
Researchers across the world are using QIAGEN Digital Insights solutions to accelerate their work in a variety of applications
Powerful insights help innovate, integrate and translate scientific results into impactful discoveries. Many noteworthy papers cite QIAGEN Digital Insights solutions and demonstrate how our tools help drive research insights and discoveries. These papers use QIAGEN Ingenuity Pathway Analysis (IPA), QIAGEN CLC and/or QIAGEN OmicSoft to help drive success. The QIAGEN Digital Insights portfolio encompasses a comprehensive, easy-to-use toolbox that ensures continuity in NGS workflow. Here, we have curated a selection of just a few recent papers to offer a sense of the diversity of the research for which QIAGEN Digital Insights solutions makes a difference.
A novel panel of differentially-expressed microRNAs in breast cancer brain metastasis may predict patient survival
First Author: Athina Giannoudis
In honor of World Cancer Day, discover how Dr. Giannoudis and colleagues at the University of Liverpool investigate differentially expressed miRNAs in breast cancer that has metastasized to the brain. See how the team uses QIAGEN IPA to identify miRNA biomarkers that may be predictive of survival. Read the paper here.
CD4+ T cell help creates memory CD8+ T cells with innate and help-independent recall capacities
First Author: Tomasz Ahrends
Read about the exciting research by T. Ahrends and colleagues at Netherlands Cancer Institute who perform a whole genome analysis to study how CD4+ T cells help generate CD8+ cytotoxic T cells. In order to identify the function and subcellular localization of the genes, the team uses QIAGEN IPA to determine which genes are differentially expressed when support from CD4+ T cells is available. Read the entire paper here.
Mediator MED23 regulates inflammatory responses and liver fibrosis
First Author: Zhichao Wang
Dive into the details of new and noteworthy research by Z. Wang and colleagues at Fudan University who study the role of MED23 in the development of liver fibrosis. Read about how the team uses QIAGEN IPA to tease out the involvement of MED23 by predicting upstream regulators of upregulated genes in a MED23 knockout mouse model, and potential targets for therapeutic intervention in liver fibrosis. Delve into the team’s research here.
Changes in DNA methylation from pre- to post-adolescence are associated with pubertal exposures
First Author: Luhang Han
To recognize Reproductive Health Day, read fascinating research by L. Han and colleagues at the University of Memphis, who perform a longitudinal study to identify epigenetic changes from pre- to post-adolescence. See how the team uses QIAGEN IPA to investigate pathways affected by the DNA methylation changes associated with puberty and environmental factors. Explore the paper here.
Genetics of glucocorticoid-associated osteonecrosis in children with acute lymphoblastic leukemia
First author: Seth E. Karol
In honor of International Childhood Cancer Day (February 14, 2020), we are highlighting this previous paper from researchers at St. Jude. Seth Karol and colleagues performed a genome-wide association study with over 2000 children and use QIAGEN IPA to identify the genetic causes of osteonecrosis that occur during glucocorticoid therapy given to children with acute lymphoblastic leukemia. Read the full paper here.
A novel mouse model of enteric Vibrio parahaemolyticus infection reveals that the type III secretion system 2 effector VOPC plays a key role in tissue invasion and gastroenteritis
First author: Hyungiun Yang
Dig into this interesting study by H. Yang and colleagues from the University of British Columbia, which reveals how V. parahaemolyticus, a bacteria commonly found in contaminated seafood, causes gastroenteritis in humans. Read how the team uses QIAGEN IPA to study the T3SS2 secretion system to show that its effectors are necessary to cause gut infection. Access the full article here.
Loss of amyloid precursor protein exacerbates early inflammation in Niemann-Pick disease type C
First author: Samuel D. Shin
For Rare Disease Day, February 28, 2020, we are highlighting research by Samuel Shin and colleagues from Loma Linda University who are studying Niemann-Pick disease type C, a lethal neurodegenerative condition, affecting one in 100,000 thousand children. Find out how the team uses QIAGEN IPA to reveal how the loss of amyloid precursor protein contributes to the neuroinflammation observed in this disease. Access the paper here.
Expression of microRNA in follicular fluid in women with and without PCOS
First author: Alexandra E. Butler
Dr. A. Butler and colleagues from Hamad Bin Khalifa University (HBKU) look at the differences in miRNA expression in follicular fluid of women with polycystic ovary syndrome (PCOS). In this recent paper, the team used QIAGEN IPA to extensively look at the differential expression of these small non-coding RNAs and identified 12 miRNAs that are involved in reproductive pathways, 12 related to inflammatory disease and 6 implicated in benign pelvic disease.
Multi-omics approach for studying tears in treatment-naïve glaucoma patients
First author: Claudia Rossi
Researchers use QIAGEN IPA to analyze the tears of glaucoma patients in this multi-'omics study to understand primary open-angle glaucoma (PAOG). In the research paper, the team performed metabolomics and proteomics analyses to identify key differences that may result in new screening options for this disease, which is the leading cause of irreversible blindness.
Longitudinal multi-omics of host-microbe dynamics in prediabetes
First author: Wenyu Zhou
Zhou and colleagues from Stanford University perform a multi-'omics study looking into the connection between host-microbiome interaction and the predisposition to type-2 diabetes. In this Nature article, read how the team uses QIAGEN IPA to search for enriched pathways to understand how changes during respiratory viral infections can lead to the development of diabetes.
Trans-ethnic association study of blood pressure determinants in over 750,000 individuals
First author: Ayush Giri
Significant research by Dr. A. Giri and colleagues from Vanderbilt University who are involved in the investigation of over 750,000 individuals for genetic variants that affect blood pressure. Discover how the team uses QIAGEN IPA to identify enriched pathways involving the 840 genes predicted to be associated with blood pressure regulation.
Fibrogenic activity of MECP2 is regulated by phosphorylation in hepatic stellate cells
First author: Eva Moran-Salvador
Discover the research by Dr. E. Moran-Salvador and colleagues from Newcastle University who study the role of MECP2 expressed by hepatic stellate cells (HSCs) in liver fibrosis. See how the team uses QIAGEN IPA to identify enriched pathways where MECP2 is involved, and how the deletion of MECP2 leads to reduced fibrosis in mice.
Immunological observations and transcriptomic analysis of trimester‐specific full‐term placentas from three Zika virus-infected women
First author: Fok-Moon Lum
Dr. F Lum and colleagues from Agency for Science, Technology and Research, Singapore look at placental development during pregnancy after a Zika virus infection. In this paper, see how the team uses QIAGEN IPA to identify eIF2 as the major canonical pathway involved in the differential gene expression pattern when compared to healthy controls.
Proteomic profiling of extracellular vesicles isolated from cerebrospinal fluid of former national football league players at risk for chronic traumatic encephalopathy
First author: Satoshi Muraoka
Dr. S. Muraoka and colleagues look at the proteomic profile of cerebrospinal fluid samples from former NFL players to understand the biology of chronic traumatic encephalopathy, a condition that affects individuals with a history of repetitive mild traumatic brain injury. In this research paper, the team use QIAGEN IPA to look at upstream regulators, pathways and functional networks of the differentially expressed proteins to identify a plausible biomarker.
Pre- and peri-implantation Zika virus infection impairs fetal development by targeting trophectoderm cells
First author: Lei Tan
Crucial research by Dr. L. Tan and colleagues from Weill Cornell Medical College strives to reveal the outcomes of a Zika virus infection during the pre- and peri-implantation stage of pregnancy. Learn how the team used QIAGEN IPA to identify two key gene networks that are strongly affected by the virus.
Multi-omics analysis identifies mitochondrial pathways associated with anxiety-related behavior
First author: Zuzanna Misiewicz
Check out this interesting paper by Dr. Z. Misiewicz and colleagues from University of Helsinki who use a multi-'omics approach to understand the molecular mechanisms behind anxiety and stress disorders. Discover how the team use QIAGEN IPA to identify certain mitochondrial genes in blood cells related to these debilitating disorders.
Microbe-host interplay in atopic dermatitis and psoriasis
First author: Nanna Fyhrquist
Interesting research by Dr. N. Fyhrquist and colleagues from the Karolinska Institute who study the interplay between the skin microbiome and skin diseases such as dermatitis and psoriasis. The team use QIAGEN IPA to identify key regulators and pathway activation in host cells to identify transcriptomic signatures for skin barrier function, tryptophan metabolism and immune activation as a basis for plausible biomarkers and targeted therapies.
Zinc chelation specifically inhibits early stages of Dengue virus replication by activation of NF-κB and induction of antiviral response in epithelial cells
First author: Meenakashi Kar
Dr. M. Kar and colleagues from Translational Health Science and Technology Institute in Faridabad, India (THSTI) perform cutting-edge immunology research using QIAGEN IPAs to understand how zinc chelation can inhibit early stages of Dengue virus by activating NFkB to induce an antiviral response in epithelial cells.
Combined transcriptome and metabolome analysis identifies defense responses in spider mite-infested pepper (Capsicum annuum)
First Author: Yuanyuan Zhang
Researchers at Wageningen University use QIAGEN CLC Genomics Workbench to study the leaf transcriptomes and metabolomes of Capsicum peppers to identify how they fight spider mite infections. Read the full story here.
Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation
First author: Shunsuke Hasegawa
Intriguing research by S. Hasegawa and colleagues at Tokyo University who investigate the role of the circadian clock in memory retrieval. See how the team uses both QIAGEN CLC Genomics Workbench and QIAGEN IPA to understand how circadian-dependent transcription factor BMAL1 contributes to loss of memory retrieval in the late afternoon. Read the details here.
Rousette bat dendritic cells overcome Marburg virus-mediated antiviral responses by upregulation of interferon-related genes while downregulating proinflammatory disease mediators
First author: Joseph Prescott
Fascinating research by J. Prescott and colleagues from the US Centers for Disease Control and Prevention (CDC) which focuses on how the immune system of the rousette bat coexists with the Marburg virus. See how the team uses QIAGEN's CLC Genomics Workbench and QIAGEN IPA to understand how bat dendritic cells downregulate immune maturation while upregulating pathogen-sensing pathways during a viral infection. Explore the topic further here.
Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade
First author: Stanislao Igor Travisano
In honor of American Heart Month, we are highlighting research by S. Travisano and team from Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Spain, who investigate the role of the Notch signaling pathway in coronary arterial development. Learn how they use both QIAGEN CLC Genomics Workbench and QIAGEN IPA to show the importance of the Dll4-Jag1-EphrinB2 signaling cascade in coronary angiogenesis. Get the details by accessing the full paper here.
Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes
First author: Sridhar Selvaraj
Exciting research from the University of Minnesota where Selvaraj et al. examine a combination of small molecules can help with the development of pluripotent stem cells into mature myotubules. Read how the team uses QIAGEN CLC Genomics Workbench and QIAGEN IPA to understand how these small molecules help with stem cell maturation.
PD-L1 blockade by atezolizumab downregulates signaling pathways associated with tumor growth, metastasis and hypoxia in human triple-negative breast cancer
First author: Reem Saleh
In this paper, read how researchers at HBKU use QIAGEN Genomics Workbench and QIAGEN IPA to understand how atezolizumab targets PD-L1 and helps in the treatment of triple-negative breast cancer, the most aggressive type of breast cancer.
Experimental evolution reveals a general role for the methyltransferase Hmt1 in noise buffering
First author: Shu-Ting You
Delve into this interesting research paper by You and colleagues from Academia Sinica in Taiwan where they study the role of methyltransferase Hmt1 in regulating noise buffering. See how the team uses QIAGEN CLC Genomics Workbench to identify Hmt-1 as a master regulator that adjusts protein noise levels in response to stressful environments.
Micro RNA transcriptome profile in canine oral melanoma
First author: Md. Mahfuzur Rahman
In this recent paper, researchers use QIAGEN CLC Genomics Workbench to understand the miRNA transcriptome profile in canine oral melanoma and how it plays a role in cancer pathogenesis. The team links their observations to three oncogenic miRNAs targets (miR-450b, 301a and 223) from a human study that were also down-regulated in canine oral melanoma and had a significant negative correlation with their respective miRNAs.
An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat
First author: Xiucheng Cui
Fascinating research out of the Ottawa Research and Development Centre where a team has developed a method to delete large segments of the genome using the CRISPR/Cas9 technique. See how they apply QIAGEN CLC Genomics Workbench to help them use an optimized Cas-9 plant codon to edit hexaploidic wheat genomes.
Genome sequence of a novel Enterococcus faecalis sequence type 922 strain isolated from a door handle in the intensive care unit of a district hospital in Durban, South Africa
First author: Christiana Shobo
December 1–7 is National Handwashing Awareness Week. Washing your hands is one of the easiest ways to prevent overuse of antibiotics and fight antimicrobial resistance. This work by Dr. C. Shobo and colleagues from the University of KwaZulu-Natal demonstrate this by using QIAGEN CLC Genomics Workbench to investigate the resistome of a novel Enterococcus faecalis found on the door handle of an intensive care unit (ICU) in South Africa. Check it out!
Human perivascular stem cell-derived extracellular vesicles mediate bone repair
First author: Jiajia Xu
Interesting research by Dr. J Xu and colleagues from Johns Hopkins University show how extracellular vesicles (EVs) derived from human perivascular stem cells (PSCs) are able to repair bone by stimulating osteoblasts just like PSCs. Discover how the team use QIAGEN CLC Genomics Server and Workbench to understand the transcriptomics of the EVs.
Comparative modulation of lncRNAs in wild-type and rag1-heterozygous mutant zebrafish exposed to immune challenge with spring viraemia of carp virus (SVCV)
First author: Valentina Valenzuela-Muñoz
Research by V. Valenzuela-Muñoz and colleagues from University of Concepción use QIAGEN CLC Genomics Workbench to discover the role of long non-coding RNAs (lncRNAs) in the infection of zebrafish with spring viraemia of carp virus.
Small extracellular vesicles convey the stress-induced adaptive responses of melanoma cells
First author: Maria Harmati
M. Harmati and colleagues from the University of Szeged use both QIAGEN CLC Genomics Workbench and QIAGEN Ingenuity Pathway Analysis to investigate how extracellular vesicles from melanoma cells convey adaptive stress responses. In their paper, they leverage these insights to illustrate how to predict different stress responses which could influence efficacy of treatments based on therapy-induced host responses.
Obesity and disease severity magnify disturbed microbiome-immune interactions in asthma patients
First Author: David Michalovich
Discover this interesting and relevant research by a team at GSK who leverage QIAGEN OmicSoft Array Studio to investigate the connection between obesity and asthma severity. They find the gut microbiome plays a significant role in these conditions. Check out the full paper here.
Reduced TRPM8 expression underpins reduced migraine risk and attenuated cold pain sensation in humans
First author: Narender R. Gavva
Researchers from Amgen use QIAGEN OmicSoft Array Studio in this study to understand how a specific allele in TRPM8 can act as a cold sensor to reduce migraine risk in humans located in colder climates.
Inhibition of mir-378a-3p by inflammation enhances IL-33 levels: A novel mechanism of alarmin modulation in ulcerative colitis
First author: Karen Dubois-Camacho
In this recent paper, researchers at the Universidad de Chile use QIAGEN OmicSoft Array Studio to study the role of miRNAs in regulating pro-inflammatory mediators such as IL-33 in ulcerative colitis, a form of inflammatory bowel disease.
PD-1hiCXCR5– T peripheral helper cells promote B cell responses in lupus via MAF and IL-21
First author: Alexandra Bocharnikov
Researchers from Harvard, Merck, Johns Hopkins and several other prominent institutions collaborate in this research paper and use QIAGEN OmicSoft Array Studio and QIAGEN IPA to understand how T peripheral helper cells contribute to B cell dysfunction in lupus.
The CSF-1-receptor inhibitor, JNJ-40346527 (PRV-6527), reduced inflammatory macrophage recruitment to the intestinal mucosa and suppressed murine T cell-mediated colitis
First author: Carl L Manthey
In this recent paper, read how researchers from Janssen Research and Development use QIAGEN OmicSoft Array Studio and QIAGEN IPA to demonstrate the involvement of macrophages in Crohn's disease and how inhibition of the CSF-1 pathway helped in attenuating the disease in mice.
Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model
First author: Haiyan Xu
Dr. H. Xu and colleagues from Merck study the ability of bone marrow cells from acute myeloid leukaemia (AML) patients to resist cancer treatment in a 3D cell culture system. Read how the team use QIAGEN OmicSoft Studio to identify unique gene expression signatures and novel genetic mutations associated with sensitivity to Ara‐C treatment in proliferating AML cells. These unique signatures could potentially be used as predictive biomarkers to determine optimal treatment regimens.
Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo
First author: Kathleen M. McAvoy
Interesting research from Dr. K. McAvoy and colleagues from Biogen and Harvard Medical School who study the genetic contribution of neuronal-specific BIN1 isoforms in late onset Alzheimer's disease. Learn how the team use QIAGEN OmicSoft Array Studio and QIAGEN Ingenuity Pathway Analysis to look at gene enrichment and activated pathways in BIN1-knockout mice to better understand this disease.
To request information on the QIAGEN Digital Insight Solutions, contact bioinformaticssales@qiagen.com.
